![]() ![]() Gel Documentation Systems Enabled for Stain-Free Gels In addition to increased accuracy, multifluorescent detection saves time, and fewer reagents are used. A gel imaging system that is capable of multifluorescent detection increases accuracy because there is no error due to inaccuracy in replicate loading or the loss of protein that occurs during stripping a blot for reprobing. This permits the detection of multiple proteins in a single sample, without requiring loading the same sample in multiple wells or stripping and reprobing the blot. Multifluorescent western blot detection allows the simultaneous detection of more than one protein on a blot. SAM provides fast, optimal results for ECL and other chemiluminescence-based protocols. In gel imaging systems with SAM capability, the system determines the best exposure time and captured image. SAM enables the capture and analysis of a time course of the development of chemiluminescent signals, unlike film-based systems, where an image is captured at a single arbitrary time point. Signal accumulation mode (SAM) is the collection of chemiluminescence data over time, allowing the measurement of chemiluminescence as it develops. Specific software modules are also offered to satisfy 21 CFR Part 11 regulatory requirements. For analyses that are often repeated, automation of the specific data acquisition, analysis, and output modes can save time and reduce errors. Multiple modes of data analysis and output provide ease of data evaluation, customized reports, sharing of data, different types of output, and generation of publication-quality images. Additionally, as your needs change and expand, our gel documentation systems have the flexibility to accommodate different sample types and detection modes. Modular design and flexible options allow you to customize a system for your specific requirements. What features might you need in a gel documentation system? Bio-Rad's gel documentation systems accommodate a wide range of samples and support different modes of detection. The choice of a gel documentation system depends on both your current and anticipated future needs. Most Bio-Rad gel documentation systems also feature our unique stain-free imaging technology, allowing rapid visualization of proteins on gels and blots without staining and destaining (Bio-Rad's TGX Stain-Free or Criterion Stain Free Precast Gels, or TGX Stain-Free FastCast Acrylamide Solutions, are also required). Our gel imaging systems can be used for detection and automated data analysis with all common modes of protein and nucleic acid staining and labeling: colorimetric, fluorescent, chemiluminescent, and radioisotopic. These gel documentation systems have advanced software, providing rapid automated data acquisition, analysis, and validation. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Bio-Rad offers a comprehensive range of imaging systems for the detection, quantitation, and analysis of proteins and nucleic acids in gels and on membranes. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Automated equipment for these steps is available to accelerate your lab workflow. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Western blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. ![]() LC-MS innovation: Improve analytical accuracy Which microbioreactor modules are best for you Which solid support is right for your oligo synthesis? 2023 ADLM Annual Scientific Meeting & Clinical Lab Expo. ![]()
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